Night Transport in Athens

Metro

The metro works daily from 5:30 am to 24:00 pm but on Friday and Saturday until 02:00 am. More Information on the Metro Website

Tram

The tram operates daily from 05:00 am to 01:00 am while on Fridays and Saturdays it operates 24 hours. More Information on the Tram Website

Buses

Each Bus line has different operating hours, but most of them work until 24:00 pm. However there are several night buses.

500 PEIRAIAS - KIFISIA (across the Green Metro Line) 00:30 - 04:30
790 GLYFADA - PERISTERI (across the Red Metro Line) 00:30 - 04:30
X14 SYNTAGMA - KIFISIA
040 SYNTAGMA - PEIRAIAS

Airport Buses work on a 24/7 basis except X94. More Information here
 

Trolleys (yellow buses)

11 A. PATISIA - N. PAGKRATI - N. ELVETIA
12 ZAPPEIO - PERISTERI (on Friday ans Saturday until 02:00)